Abstract: The aim of this study was to determine morphometric features of lumbar segments of the spinal cord of horses. Spinal cords of six horses from different breeds and sexes were used. Lumbar parts of the cords were dissected out from vertebral columns and segmentation was performed. Macroscopic measurements were done on the segments and then routine histological procedure was applied. Segments were sliced transversally at 3.8 mm thickness. Samples were cut at 10 μm thickness and stained with May-Grunwald-Giemsa. Slides images were used by stereo investigator. Diameter measurements were carried out and area and volume calculations were performed by using Cavalieri principle. The weight, length and volume ratios of the lumbar parts to spinal cord were 14.7, 13 and 14%, respectively. A decrease in lengths of the segments from L₁ to L₆ was observed. In the microscopic analysis, L₄ and L₅ had the biggest transverse diameters however L₆ had the largest vertical diameter. L₅ had the largest values of cross-sectional and white matter area however L₆ had the largest value of gray matter. Volumes of all segments except for L₆ were similar to each other and the largest volume of gray matter and the largest volume of white matter were observed in L₅ and L₃, respectively. It was determined that the volume ratio of gray matter to the volumes of segments increased from L₁ to L₆ whereas volume of white matter was decreased in those segments. In conclusion, the data obtained will contribute to the morphology of related region and the further studies.