Authors : Zuoyong Luo, Baojie Wang, Mei Liu, Keyong Jiang and Lei Wang
Abstract: Different amounts of vitamin E supplementation were added to feed sea cucumber (Apostichopus japonicus Selenka) juveniles (2.5±0.15 g) testing to reduce nitrite stress. Commercial feed was used as the control diet, 3 experimental diets containing vitamin E supplementation (150, 250 and 350 mg kg-1 diet), respectively was designed to experiment for 45 days. The specimens were exposed to three different concentrations (0.5, 1.0 and 1.5 mg L-1) of nitrite for 4, 8 and 12 h at four different time points (0, 15, 30 and 45 days). Hydroxyl free radical (-OH), Malondialdehyde (MDA), Total Antioxidant Capacity (T-AOC), Superoxide Dismutase (SOD) and Catalase (CAT) were measured. The control group’ level of -OH and MDA turned higher whereas T-AOC turned lower and SOD and CAT activity elevated after exposed to nitrite solution. The experimental group’ level of -OH and MDA turned lower whereas T-AOC turned higher. The results suggested vitamin E supplementation diets could reduce nitrite stress response and increase the antioxidant capacity. The optimal amount of vitamin E supplementation ranged from 180-260 mg kg-1 and number of feeding days ranged from 33-35 days were obtained according regress equations.
Zuoyong Luo, Baojie Wang, Mei Liu, Keyong Jiang and Lei Wang, 2014. Effect of Dietary Vitamin E Supplementation on Oxidative Stress Response of Acute Nitrite Exposure in Sea Cucumber (Apostichopus japonicus Selenka) Juveniles. Journal of Animal and Veterinary Advances, 13: 77-88.