Authors : Jee Eun Han, Ji Hyung Kim, Jin Woo Jun, Hyoun Joong Kim, Sae Kil Yun, Ji Young Chai and Se Chang Park
Abstract: A total of 28 Aeromonas strains obtained from water and diseased fish in South Korea were examined for virulence gene screening. Strains were tested by multiplex PCR for the presence of 2 β-hemolysins genes and 3 Type III Secretion System (TTSS) genes. All A. hydrophila strains carried both aerA genes and ahh1 genes and 5 out of the 8 A. sobria strains carried asa1 genes for β-hemolysins. Additionally, 11 strains carried aopB, aopD or ascV genes for TTSS (aopB gene was detected in 7 A. hydrophila strains and 4 A. sobria strains, aopD gene was detected in 5 A. hydrophila strains and 1 A. sobria strains and ascV gene was detected in 4 A. hydrophila strains and 3 A. sobria strains). Interestingly, some sequence of TTSS genes from tested A. hydrophila showed strong genetic variation to those of the reported A. hydrophila, the aopB genes of strain (SNUFPC-A3) was 94% homologous to A. salmonicida (Accession No. AJ616218.2) those of strains (SNUFPC-A10 and CASAH2) were 98% homologous to A. sobria (Accession No. EF215451.1) and the aopD genes of strain (CASAH2) was 98 homologous to A. sobria (Accession No. EF215451.1).
Jee Eun Han, Ji Hyung Kim, Jin Woo Jun, Hyoun Joong Kim, Sae Kil Yun, Ji Young Chai and Se Chang Park, 2014. Distribution of Virulence Associated Genes and Type III Secretion System Genes in Motile Aeromonas. Journal of Animal and Veterinary Advances, 13: 828-833.