Authors : Xian Guo, Jie Pei, Min Chu, Hongbo Wang, Xuezhi Ding and Ping Yan
Abstract: Proteome refers to the entire protein set encoded by a single genome. To understand the mechanisms underlying the seasonal reproduction of yak at the protein level and to examine the changes of protein composition in the yak follicular fluid and plasma, the differential protein and its composition in the yak follicular fluid and plasma have been identified by two dimensional electrophoresis (2DE) and Mass Spectrometry (MS). The follicular fluid and plasma from yak on Qinghai Plateau were subjected to 2DE and silver staining. The gel image was analyzed by image master 2D Platinum Software followed by Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF/TOF)TM MS identification. The proteins of high abundance were removed using ProteoExtract Albumin/Immunoglobulin G (IgG) Removal kit. Gel images with high resolution, clear background and good reproducibility from follicular fluid and plasma were obtained by 2DE. Comparison between images from follicular fluid and plasma showed 24 differentially expressed proteins, of which two were up-regulated and twenty two were down-regulated. MS analysis identified eight proteins and five unknown proteins. The proteomic images were successfully separated and identified differential proteins between follicular fluid and plasma in the yak which provided experimental evidence for the mechanisms of yak follicular development and the understanding of microenvironment for oocyte development.
Xian Guo, Jie Pei, Min Chu, Hongbo Wang, Xuezhi Ding and Ping Yan, 2015. The Two Dimensional Electrophoresis and Mass Spectrometric Analysis of Differential Proteome in Yak Follicular Fluid. Journal of Animal and Veterinary Advances, 14: 75-80.