Authors : Krzysztof Marycz, Joanna Szydlarska, Zuzanna Strek and Christine Weiss
Abstract: Regular exercises not only contribute to the growth and maintenance of lean muscle mass but also bring a wide range of health benefits. On the other hand excessive training may cause oxidative stress, inflammation and metabolic abnormalities. The risk of disorders is particularly high among the young athletic horses and often leads to elimination from the sports career. The aim of the study was to investigate how exercises combined with anti-oxidants and nucleotides supplementation influenced on muscle pro-inflammatory cytokine activity, mitochondrial biogenesis and selective mitophagy. We evaluated mitochondrial morphology and distribution in skeletal muscle using transmission electron microscopy. Creatine kinase aspartate transaminase and superoxide dismutase activities were estimated by means of commercially available methods. Moreover, we determined the mRNA levels of muscle-related genes using a modified polymerase chain reaction. Our research revealed that there is a positive correlation between prolonged physical exertion in combination with well-conceived nourishment and muscle mitochondrial biogenesis as well as dynamics. Under the influence of describing dietary strategy we observed reduction of creatine kinase and asparate transaminase levels in peripheral blood. We also noticed that muscle apoptosis and inflammation in experimental groups was lowered, when compared to the control group. Obtained results indicate that physical activity combined with dietary enriched by anti-oxidants and nucleotides might increase mitochondrial number, function and efficiency in addition to reducing systemic inflammation and apoptosis at the mRNA level which might be particularly important for sport horses breeders.
Krzysztof Marycz, Joanna Szydlarska, Zuzanna Strek and Christine Weiss, 2018. The Effect of Dietary Nucleotides Combined with Anti-Oxidants Supplementation on Equine Muscles Inflammation and Autophagy. Journal of Animal and Veterinary Advances, 17: 51-60.