Authors : Wan Shahriman Yushdie Wan Yusoff, Intan Nur Shahfiqah Baharum, Evana Kamarudin, Mohd Fahmi Mastuki and Mohd Nazri Abu
Abstract: Food contaminants such as vegetable particles in cytology specimens can cause misdiagnosis by mimicking the cells and microorganisms. Misdiagnosis can contribute to false positive or false negative result which then whether will cause delays in diagnosis or an unnecessary treatment thus led to morbidity. The objective of this study is to describe the morphology of traditional salads cells in Malaysia and to identify the mimicking characters of these cells with normal, abnormal and microorganism from cytology specimens. Commonly consumed traditional salads in Malaysia were selected and homogenized using mortar and pestle. This is to resemble chewing affect by breaking down the samples into small particles. Each samples were smeared onto glass slides using back-to-back method. Prepared slides were stained with PAP and MGG stains. The cells were observed and images were recorded. Traditional salads contaminants can resemble Cocobacillus sp., Trichomonas vaginalis, Candida sp. spores, parasites ova, squamous epithelial cells, squamous glandular cells and HSIL. As a conclusion, most of traditional salads cells cytomorphology in this study can mimic human cells and microorganisms in cytology specimens. These finding will provide reference in cytology diagnostic to minimize the rate of misdiagnosis which reassure proper patient treatment.
Wan Shahriman Yushdie Wan Yusoff, Intan Nur Shahfiqah Baharum, Evana Kamarudin, Mohd Fahmi Mastuki and Mohd Nazri Abu, 2016. Malaysian Traditional Salads Particles Resembling Microorganisms and Cells in Specimens for Cytology. Journal of Engineering and Applied Sciences, 11: 2118-2121.