Abstract: Microfabrication technique based on microelectronic technology has the capability to produce microfluidic devices in micro or nano-meteric scale but this technique involves with costly process and toxic chemicals. In this study, we proposed the use of patterned vinyl adhesive template to produce a flow focusing microfluidic device on a work bench that was applied to generate microbeads of calcium alginate for microencapsulation of cells. In this researche an infusion pump of high flow rates (1000-5000 μL/min) was developed to be used together with a commercial syringe pump that could pump the fluid to emulsify the continuous and disperse phases leading to the production of microbeads. Microbeads of calcium alginate in the range of 200-800 μm was successfully produced using this simple, environment friendly and efficient technique.
Chin Fhong Soon, Hiung Yin Yap, Mohd Khairul Ahmad, Mohd Zainizan Sahdan, Kian Sek Tee, Sayed Ali Khagani and Mansour Youseffi, 2017. Environmental Friendly Flow Focusing Microfluidic Device and Pump System for Microencapsulation of 3D Cells. Journal of Engineering and Applied Sciences, 12: 6928-6933.