Abstract: The protein extraction using saline treatment method (NaCl) from superworm (Zophobas morio) was investigated. The effect of four independent variables, namely reaction time (10-20 min), pH (10-12), rotation speed level (6-9) and NaCl concentration on the protein extraction was designed by Box-Behnken and analysed using Response Surface Methodology (RSM). The protein extracted was quantified and determined using Bradford protein assays. A second-degree equation for independent and response variables was obtained. The optimum conditions for the highest protein extraction (49.20 mg/mL) were found to be at 0.3 M NaOH in 20.0 minutes reaction time with a rotation speed level of 6 and pH 12. Longer reaction time and higher pH shows significant increase for protein extraction from superworm. The results obtained will contribute to the information on the productivity and quality of the protein yield extracted from superworm.
Tan Kian Meng, Noor Akhmazillah Mohd Fauzi and Angzzas Sari Mohd Kassim, 2017. The Extraction of Protein from Superworm (Zophobas morio) using Saline Treatment (NaCl) Method. Journal of Engineering and Applied Sciences, 12: 6953-6957.