Abstract: The aims of this study were to determine the similarity and difference of Eurycoma longifolia (EL) and Polyalthia bullata (PB) on body weight, reproductive organs weight and regulation of potent androgen, testosterone and prohormone, De Hydro Epi Androsterone (DHEA) in Sprague-Dawley rats. Adult male Sprague-Dawley rats weighing 150-250 g were divided into 3 groups (n = 6) where control group received purified water, EL group and PB group received 400 mg/kg of treatment, respectively. The daily body weight of each rat was recorded during the treatment period. After 14 days of treatment (day 15), all rats were anaesthetised using ketamine/xylazine followed by cardiac puncture for blood collection. Rats were dissected and target organs were removed and weighed. Serum was obtained to determine testosterone and DHEA levels. Data was analysed using SPSS Version 23.0. There was a significant increase in mean percentage changes of body weight of EL compared to control group (p = 0.003) while a significant decrease in PB 400 mg/kg group compared to control group (p = 0.04). Treatment of EL has caused a decrease in right testes weight compared to control (p = 0.026) while treatment of PB has caused a decrease in right epididymis weight compared to control (p = 0.04). Both treatments have no effect on prostate and seminal vesicles weight of SD rats. Treatment of EL has caused a significantly increased serum testosterone concentration compared to control and PB (p = 0.04, p = 0.03), while there is no difference in serum DHEA concentration between all groups. In comparison of the in vivo effect of known aphrodisiac EL and PB, it is concluded that EL has more prominent effect in percentage of body weight changes, testis weight and serum testosterone level compared to PB. Further study is essential to determine other effects of these two plants on testes histology, androgens level and expression levels of steroidogenic enzymes.
Mashani Mohamad, Hannis Fadzillah Mohsin and Gurmeet Kaur Surindar Singh, 2017. A Study on the Effects of Eurycoma longifolia and Polyalthia bullata on Reproductive Organs and Androgens of Male Sprague-Dawley Rats. Journal of Engineering and Applied Sciences, 12: 6994-6999.