Plant Sciences Research

Year: 2020
Volume: 12
Issue: 1
Page No. 1 - 6

Phytochemical Screening and Thin Layer Chromatographic Analysis of Arachis hypogaea L. Seed Extracts

Authors : Tsegu Kiros and Negussie Bussa

Abstract: Arachis hypogaea L. belongs to the genus Arachis, family Leguminoseae is a legume plant. The seed of Arachis hypogaea contains various bioactive phytochemicals in addition to the identified oil constituents and other sources of nutrition. However, reports regarding the role of the seed part of Arachis hypogaea as source of phytochemicals and other pharmaceuticals were very minimal. The objective of this study was therefore to investigate the presence of various phytochemicals and analysis thin layer chromatographic profiles of crude seed extracts of Arachis hypogaea. The ground powder of dried seed of Arachis hypogaea was extracted using petroleum ether, methanol and aqueous, separately by putting on an incubated orbital shaker for 24 h. Each extracts were then filtered through Whatman No. 1 filter paper, concentrated using rotary vacuum evaporator and calculated the percentage yield. Standard qualitative procedures were employed to identify the major phytochemical constituents. The TLC profiles of each crude seed extracts were observed through UV-lamp followed by iodine vapour. Besides to this, fluorescence analysis of all the crude extracts under UV-lamp and ordinary light has been carried out. The phytochemical analysis of different solvent extracts of Arachis hypogaea seed studied herein revealed a strong, moderate and weak presence of alkaloids, glycosides, tannins, flavonoids, phenols, sterols, fats, oils, saponins and quinines. And from the TLC profile, two spots with Rf values 0.13 and 0.86 were observed in common in all the solvent extracts of Arachis hypogaea seed. The present study suggests that the seed of Arachis hypogaea L. can be used as food supplements in everyday diet andmay also useful in pharmaceutical industry being the phytochemicals it contains can be used as precursors for the isolation and characterization of pure bioactive compounds employed as ingredients in drug formulation.

How to cite this article:

Tsegu Kiros and Negussie Bussa, 2020. Phytochemical Screening and Thin Layer Chromatographic Analysis of Arachis hypogaea L. Seed Extracts. Plant Sciences Research, 12: 1-6.

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