Abstract: In the present study, the effects of heavy metals Lead (Pb), Cadmium (Cd) on the liver enzymes Glutamate Oxalate Transaminase (GOT), Glutamic Pyruvate Transaminase (GPT) and testosterone hormone concentration heavy asphalt workers were investigated. Research material consists of blood samples taken from Van highway asphalt workers and rural community (Bardakci). The research was conducted with attention paid to personal traits. Results of lead, cadmium, enzyme activities and hormone content in the blood samples of workers and rural people are interpreted by variance analysis with uni-variety method in Spss package software. According to the results, GOT amount in highway asphalt workers was 23.27±l.09 U L^-1 while it was 23.82±1.75 U L^-1, GPT amount in highway asphalt workers was 23.82±1.75 U L^-1 while it was 20.48±1.61 U L^-1 in the control group. Pb amount was significant at the rate of 14.64±0.64 μg/l00 mL in asphalt workers and 5.04±0.60 μg/l00 mL and p<0.0l in the control group; Cd amount was significant at the rate of 0.l6l±0.028 μg/l00 mL in the asphalt workers and 0.038±0.026 μg/l00 mL and p<0.01 in the control group with the amounts and significance as 0.283±0.032 μg/l00 mL in smoking workers and 0.038±0.021 μg/100 mL and p<0.0l in non-smoking workers; the amount and significance of testosterone was found as 631.38±41.09 ng dL^-1 in non-smoking asphalt workers and 388.65±29.05 and p<0.0l in smoking workers; the amount of testosterone in non-smoking asphalt workers was 657.38±36.04 and 450.05±30.63 dL^-1 in smoking asphalt workers with p<0.01 significance.