Abstract: House gecko has become almost domesticated in every other homes in Nigeria especially Ibadan metropolis where this study was carried out due to its reduced flight zone. A total of 25 animals comprising 11 females and 14 males were collected for the study. Fresh blood was taken from the ventral abdominal vein into heparinised syringes for haematological analysis. Result revealed plasma protein density (4.709±0.48 g dL^-1), haemoglobin concentration (8.127±2.18 g dL^-1), red blood cell count (2.287±0.62) *10⁶ μL^-1, white blood cell count (1.563±3181.85) *10⁶ μL^-1, lymphocytes (68.273±3.17) *10³ μL^-1 and eosinophils (4.546±1.86) *10³ μL^-1 and of which values of the females were slightly higher than those of the male house geckos while the PCV (24.000±6.59%), Platelets (1.647±41403.12) *10⁶ μL^-1, heterophils (27.182±4.58) *10³ μL^-1 and monophils (2.636±0.50) *10³ μL^-1 were slightly higher in males than in female house gecko studied in the five local government areas LGAs in Ibadan metropolis. There were no significant differences in the plasma protein, packed cell volume, hemoglobin concentration, red blood cell counts, platelets, lymphocytes, heterophils, monophils and eosinophils values of both male and female house geckos across the five LGAs while in contrast there was a significant difference in the WBC values of the male and female house geckos across the five LGAs of Ibadan.