Authors : Yanying Zhang, Qiumei Shi, Shuqin Cheng, Guisheng Gao, Hai Fang, Guangping Gao, Yuqin Liu, Cuizhen Chen and Qiang Wang
Abstract: A total of 54 samples including duodenum, small intestine contents, lymphonodi mesenterici and diarrhea feces were collected from pigs died of diarrhea in Hebei Province of China in 2009~2011. These samples were examined for the presence of E. coli and serotype identification. High pathogenicity island was detected from E. coli isolates. The isolation and identification of O serotype of E. coli were conducted by common method, fyuA and irp2 genes were detected using PCR. The 54 E. coli strains referred to ten serotypes, O38 was the dominant serotype whose proportion was 51.5% (17/33). The positive rate of fyuA gene was 24.4% (11/45), irp2 was 42.2% (19/45), irp2 and fruA was 13.3% (6/45).
Yanying Zhang, Qiumei Shi, Shuqin Cheng, Guisheng Gao, Hai Fang, Guangping Gao, Yuqin Liu, Cuizhen Chen and Qiang Wang, 2013. Serotype Identification and Detection of Related Genes of HPI Pathogenic Island of Escherichia coli Isolated from Pigs in Hebei Province of China. Veterinary Research, 6: 99-104.