Abstract: A descriptive type of research was conducted in July 2006 to determine the factors promoting self-motivating behaviours at work among the middle level management staff (Managers) of the Ministry of Agriculture (MoA), Botswana. The study included all the 30 middle level managerial staff of the Ministry of Agriculture. A structured questionnaire was used to collect information on the personal characteristics of the respondents. Also, the questionnaire consisted of statements anchored on a 5 point likert type rating scale. The statements were developed from literature and represented general self-motivating behaviours among staff members of any organization. The statements were validated and the reliability estimate calculated and found to be 0.93, indicating a good degree of reliability. Frame error and selection error were catered for; sampling error was not a threat since all the target population for the study 30 participated in the study. The data collected were analysed using frequencies and percentages to describe the personal characteristic of the respondents. means, standard deviations and ranks were used to describe the factors promoting self-motivating behaviours at work place among the respondents. Correlation coefficients were used to describe the relationship between some selected personal characteristics and of the managers towards factors promoting self-motivating behaviours at work. Results revealed that Male dominated the managerial cadre of the MoA staff. Also, the managers were mostly Diploma graduates, having between 11-25 years of experience and mostly within the age bracket of 31-40 years. Majority of the managers responded favourably in varying degrees to the factors promoting self-motivating behaviours at work. Also, most of the factors promoting self-motivating behaviours attracted a negligible relationship between the personal characteristics of the managers and the self-motivating behaviours examined in the study.