Authors : F. Sahnouni, A. Matallah-Boutiba, D. Chemlal and Z. Boutiba
Abstract: In spite of overwhelming information about Lactic Acid Bacteria (LAB), only a few studies are available for the LAB of marine environment. The purpose of the present study was the characterization of new strains of LAB from gastrointestinal tract of coastal fish; sardine (Sardina pilchardus) and bug (Boops boops) with potential application as biopreservatives. A total of 67 isolates were isolated of which 16 isolates displayed antibacterial activities against S. aureus, E. coli, P. aeruginosa, K. pneumoniae, B. cereus, Salmonella sp. and E. faecalis. Also an antifungal activity was detected against Fusarium oxysporum and Aspergillus sp. The strains selected for their antimicrobial activity were identified on the basis of phenotypic characters including API system as: L. lactis ssp. lactis, L. lactis ssp. diacetylactis, Leuconostoc sp. and Lactobacillus plantarum. Three strains were screened for the study of their antibacterial compounds: L. lactis ssp. lactis MT, L. lactis diacetylactis BT4 and L. lactis ssp. diacetylactis BL10. As a result it was observed that inhibitory activities of all three LAB strains were due to bacteriocin-like substances. This antimicrobial activity was inactivated by the addition of proteinase K, α-chymotrypsin but not by lipase. All the strains were no hemolytic showed no particular antibiotic resistance profile able to hydrolyze casein whereas none of them is found to possess lipolytic activity, however the majority produces biogenic amines.
F. Sahnouni, A. Matallah-Boutiba, D. Chemlal and Z. Boutiba, 2012. Characterization of Technological Properties of Lactic Acid Bacteria Isolated from Intestinal Microbiota of Marine Fish Caught in the Coast of Oran Algeria. Agricultural Journal, 7: 81-87.