Abstract: Due to the harmful effects of job stress, the study has been conducted with the aim of rating and comparing organizational factors affecting job stress of manger faculty members and non-manager faculty members of Ferdowsi University of Mashhad based on Luthans Model. The current study is applied objectively and in terms of nature and method is descriptive-survey. The study population consisted of all faculty members of Ferdowsi University of Mashhad. The sample size was estimated 117 people according to the formula of determining sample size. Sampling method is stratified random sampling that using proportional allocation formula, the participants were selected. Job stress questionnaire of Luthans was used for collecting the field data and its reliability and validity was investigated and confirmed. To test the hypotheses, two-sample student t-test method is used. The research results showed that total internal factors of Luthans Model affect job stress of faculty members but the order of factors affecting job stress of manger faculty members and non-manager faculty members is different so that the order of factors for manager faculty members includes organizational processes; organizational policy; organizational structure and physical conditions and order of factors for non-manager faculty members includes; organizational structure; organizational policy organizational process and Physical conditions. In this study also was specified, gender, education of faculty members not affect job stress. In the end, the required proposals are presented to reduce job stress of manger faculty members and non-manager faculty members.