Authors : R.P. Sumarno, S. Winarsih, S. Hidayat and Y.W. Utami
Abstract: Shigellosis is still a big problem in developing countries. Prevention using a vaccine so far there has not be any suitable vaccine. Molecule adhesion of bacteria can serve as a basic component of the vaccine. S. dysenteriae sub-unit pili protein which has MW 7.9 and 48.9 kDa molecule is adhesive. Rabbit Ileal Loop Model can evaluate bacterial diarrhea. Similarly, Mice Legated Ilea Loop Model can be used to evaluate the bacterial diarrhea as well. The study are to clarify that mice which immunized with S. dysenteriae sub-unit pili molecular weight 7.9 and 48.9 kDa as an adhesive molecule can protect moving solution into lumen intestine and to prove that Mice Legated Ilea Loop Model can be used to show diarrhea protection. The study was conducted by protectively test with post control study design and Mice Legated Ilea Loop Model. The first group without immunization was used as a control group. The second group was immunized with S. dysenteriae sub-unit pili protein which has molecular weight 7.9 kDa. Third group was immunized with S. dysenteriae sub-unit pili protein which has MW 48.9 kDa. The last group was immunized with combination of S. dysenteriae sub-unit pili proteins which has molecular weight 7.9 and 48.9 kDa. Data were analized by ANOVA and Tuckey test. The significant differences in intestines weight in the control group between second, third and last group. There was no different effect in the addition of different proteins. S. dysenteriae sub-unit pili protein which has MW 7.9 and 48.9 kDa can be used as candidate of vaccine shigellosis and the MLIL test can be applied for studying bacterial diarrhea.
R.P. Sumarno, S. Winarsih, S. Hidayat and Y.W. Utami, 2015. Shigella dysenteriae Pili Proteins as an Adhesive Molecule Can Protect Moving Solution by Using Mice Legated Ilea Loop Model. International Journal of Tropical Medicine, 10: 1-4.