Authors : S?kr? Beydemir and ?lhami G?lcin
Abstract: The effects of streptomycin sulfate, gentamicin sulfate and sodium dipyrone have been investigated on rat erythrocyte carbonic anhyrase enzyme (CA) (EC 4.2.1.1) in vitro. For this purpose, rat erythrocyte carbonic anhyrase (CA) was purified. Rat erythrocyte carbonic anhyrase (CA) was purified. The purification steps comprised high-speed centrifugation, Sepharose-4B-L tyrosine-sulfanilamide affinity gel chromatography and dialyze. The yield was 44.4 % and the enzyme was found to be specific activity of 13873.2 EU/mg proteins. The overall purification was about 629.74-fold. Temperature of +4?C was maintained during the purification process. Inhibition or activation effects of three different medical drugs on rat CA were determined using the Esterase method by using spectrophotometer at 348 nm. Streptomycin sulfate showed highly inhibition effect the enzyme activity in in vitro conditions. I50 value of the streptomycin sulfate was determined as 32.8 mM by means of these graphs Activity %-[Drug] graphs. However, gentamicin sulfate showed in vitro inhibition effect on CA esterase activity up to 2.8 mM concentration, and then it was observed activation effect on the enzyme activitiy. Beside, sodium dipyrone has not any effect on rat erythrocyte CA.
S?kr? Beydemir and ?lhami G?lcin , 2003. The In vitro Effects of Some Medical Drugs on Rat Carbonic Anhydrase . Journal of Animal and Veterinary Advances, 2: 143-146.