Abstract: To investigate the effects of long-term somatoliberin on changes in plasma GH, LH and progesterone and blood metabolites in growing female buffalo calves, twelve calves within the age group of 6 to 8 months of age were divided into two groups (treatment and control groups) of six each in such a way that their mean body weight did not differ (P>0.01) and were fed as per Kearl standard (1982) for growing buffaloes. Treatment group buffaloes were administered i.v. with synthetic bGRF [ somatoliberin; bGRF (1-44)-NH2 ] @ 10Fg/100 Kg. body weight at fortnight interval till 18 injections were completed (9 months). Blood samples were collected at fifteen days interval one month prior to and two month post-treatment for estimation of plasma GH, LH and progesterone and blood metabolites viz. non-esterified fatty acids (NEFA), alpha amino nitrogen and glucose. During pre-treatment period, plasma GH, LH and progesterone and blood metabolites did not differ (P>0.05) between the groups. Post-somatoliberin administration at the end of first month, the plasma GH and LH showed a higher trend (P<0.001) in somatoliberin treated buffaloes over untreated controls and this trend continued even in post-treatment period. Increasing trend (P<0.01) of plasma LH with advancing age of the calves was also recorded in both the groups. Unlike untreated controls, a definite up and rise trend of plasma progesterone was recorded in somatoliberin treated group during treatment period. Plasma NEFA, alpha amino nitrogen and glucose were found to be higher (P<0.05) in treatment than control group. Results indicate that long-term exogenous somatoliberin administration sustained a higher level of plasma GH even after cessation of treatment without showing any sign of refractoriness in this species. The buffaloes treated with somatoliberin may approach puberty earlier as they would reach the >critical= body mass earlier, is also reflected by higher plasma progesterone and LH concentrations. However, detailed studies are required to ascertain the hypothesis.