Authors : A. E. Karrar , M. E. H. Mohamed , A. A. Majid , S. M. M Elamin , M. M. Salih and I. E. Aradaib
Abstract: The Sudanese isolates of epizootic hemorrhagic disease virus (EHDV) serogroup were characterized using electron microscopy, agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE). The virus measures about 70-80 nanometers in diameter as determined by electron microscopy. The double stranded RNA (dsRNA) genome segments of the Sudanese serotypes of EHDV serogroup were analysed by agarose gel and sodium dodecyl sulphate (SDS)- polyacrylamide gel electrophoresis (SDS-PAGE). The dsRNA genome segments profiles of Sudanese EHDV serotypes 4 and an untyped isolate designated (EHDV-318) were compared with that of North American EHDV serotypes 1. Both systems (agarose gel and SDS-PAGE) showed 10 segments, which is characteristic pattern of EHDV serogroup. The agarose gel showed identical genome profiles for all Sudanese and North American EHDV serotypes 1. However, SDS-PAGE system was able to detect genetic variation between and among Sudanese and North American EHDV serotypes. The result of this study suggested that, the electron microscopy and the agarose gel electrophoresis could be used as supportive or complementary methods to facilitate tentative diagnosis of EHDV infection in susceptible ruminants. In addition, SDS-PAGE could be used to detect genetic variations between serotypes of EHDV from the African or North American continents.
A. E. Karrar , M. E. H. Mohamed , A. A. Majid , S. M. M Elamin , M. M. Salih and I. E. Aradaib , 2003. Characterization of Epizootic Hemorrhagic Disease Virus Serogroup Recovered from Sentinal Cattle at Shambat, Central Sudan . Journal of Animal and Veterinary Advances, 2: 611-614.