Authors : Muna O. Elhaj and S.M. Elsanousi
Abstract: Six local Sudanese isolates of S. aureus subsp. anaerobius which were isolated from sheep abscesses were compared with the reference strain of S. aureus subsp. anaerobius ATCC 35844 DSM (Deutsche Sammlung von Microorganismen und Zelkulturen, Braunschweig, Germany) No. 20714 in their protein profiles to choose the candidate local isolate for vaccine production.Vertical electrophoresis was used to separate protein of cell lysates and supernatants of the local Sudanese isolates and the reference strain of S. aureus subsp. anaerobius. It was found that the electrophoretic protein pattern for all local isolates and the reference strain resembles each others to a greater extent and many bands are common in all strains. Cell lysates of all local isolates (except isolate 5) and the reference strain had protein bands ranged between 19-24 with molecular mass ranging from 53.35-113.84 KDa. Isolate 5 have 17 protein bands with molecular mass ranging from 53.62-74.89 KDa. All local isolates and the reference strain had no protein bands in their supernatants, except isolate 2 which has 5 protein bands in its supernatant. Most of the local isolates are the same as the reference strain, two local isolates have minor differences compared to the reference strain.
Muna O. Elhaj and S.M. Elsanousi , 2005. SDS-Polyacrylamide Gel Electrophoresis for Comparison of S. aureus subsp. anaerobius Local Sudanese Isolates and the Reference Strain ATCC 35844. Journal of Animal and Veterinary Advances, 4: 962-964.