Authors : S.O. Sarah , M. Zamri-Saad , Z. Zunita and A.R. Raha
Abstract: Pasteurella multocida has been recognised as an important pathogen of animals that causes a wide spectrum of diseases, collectively termed as pasteurellosis. The bacteria possess several housekeeping genes that maintain the viability of the bacteria. One of the housekeeping genes is the gdhAgene that serves as a major link between carbon and nitrogen metabolism. The full length gdhA gene of P. multocida B:2 was found to be 1180bp while the functional fragment of the gene was 652bp. The gene was successfully sequenced and later cloned into E. coli pCR 2.1-TOPO vector.
S.O. Sarah , M. Zamri-Saad , Z. Zunita and A.R. Raha , 2006. Molecular Cloning and Sequence Analysis of gdhA Gene of Pasteurella multocida B:2. Journal of Animal and Veterinary Advances, 5: 1146-1149.