Abstract: The most important criteria on scientific studies at honeybee colonies are colony population development. A commonly used division is to estimate capped and uncapped brood areas. At this point of view, it is important to measure brood area in colony beside frame of bees. Brood area that contained eggs, larvae, and pupae on the combs in each bee colony has been measured as its size. Assessing a whole colony for strength provides a very good snapshot in time of colony size, brood production and food status. The estimation technique may take a bit of getting used to, but with experience it can be done quickly and accurately. To estimate the total area of capped worker brood cells in the colony can be used usually a 1 x 1 inch wire grid placed over the brood comb to estimate the total square inches of brood for each side of the comb. Another alternative method is digital photography. Measurements of worker brood area are determined by measuring sealed brood to the nearest cm² using Adobe Photoshop® CS2 9.0. This method based on estimating capped and uncapped brood. Colonies with new comb produced a greater area (cm²) of brood, a greater area (cm²) of sealed brood.