Abstract: The objective of this study was to investigate the effects of soluble and insoluble non-starch polysaccharides isolated from wheat bran on endogenous amino acid flow at the terminal ileum of growing rats using the protein-free method. In experiment 1, 20 Sprague-Dawley rats (200 ± 0.9 g), were fed either a protein-free, low fiber diet based on cornstarch, sucrose and soybean oil or a similar diet containing 50 g kg 1 of soluble non-starch polysaccharides added at the expense of cornstarch. In experiment 2, 40 Sprague-Dawley rats (198 ± 0.8 g), were randomly allotted to either the protein-free, low fiber diet or similar diets containing either 50 g kg 1 insoluble non-starch polysaccharides, 50 g kg 1 soluble non-starch polysaccharides or a combination of 50 g kg 1 soluble and 50 g kg 1 insoluble non-starch polysaccharides added at the expense of cornstarch. Chromic oxide (6 g kg 1 diet) was included in all diets as an indigestible marker. Both experiments lasted 8 d. The rats were killed on day 8 and the digesta contained in the final 20 cm of the ileum was obtained for assay. Compared with the protein-free basal diet, adding 50 g kg 1 soluble non-starch polysaccharides significantly (p<0.01) increased the endogenous flow of amino acids and nitrogen at the terminal ileum (both experiment 1 and 2). Including 50 g kg 1 of insoluble non-starch polysaccharides also significantly increased (p<0.05) the ileal flows of amino acids and nitrogen. The effects of the soluble non-starch polysaccharides were 22-85% greater than those of the insoluble non-starch polysaccharides.
Y.H. He , W.Q. Lu , D.F. Li , H.L. Zhang and H.Q. Jiang , 2006. Effects of Soluble and Insoluble Non-starch Polysaccharides Isolated from Wheat Bran on Endogenous Amino Acid Loss at the Terminal Ileum of Growing Rats. Journal of Animal and Veterinary Advances, 5: 143-149.