Authors : A.C. Rodriguez-Aguirre , C.F. Rodriguez-Ortega , A.M. , Nevarez-Garza , L.E. Rodriguez-Tovar , G. Hernandez-Vidal and R. Ramirez-Romero
Abstract: Mast cells numbers and reactivity status based on degranulation was determined in the skin of diabetic rabbits subjected to a local Shwartzman reaction. Diabetes was induced by a single intravenous injection of alloxan. The local Shwartzman reaction was induced seven days after by two inoculations of Salmonella typhimurium lipopolysaccharide. Preparatory injection was received in the skin and 24 h later; the provocative injection was administered intravenously. Rabbits were killed at the next day. Skin samples were fixed in Carnoy’s and mast cell were identified employing a low pH toluidine blue stain. Results showed that Shwartzman reaction influences dramatically the increment and reactivity of mast cells independently from diabetes. In this model of acute inflammatory response diabetes has no influence on mast cells.
A.C. Rodriguez-Aguirre , C.F. Rodriguez-Ortega , A.M. , Nevarez-Garza , L.E. Rodriguez-Tovar , G. Hernandez-Vidal and R. Ramirez-Romero , 2008. Short-Term Diabetes has no Influence on Mast Cell Infiltration and Reactivity During the Local Shwartzman Reaction. Journal of Animal and Veterinary Advances, 7: 258-262.