Abstract: In the present study, effects of ovsynch-CIDR protocol was compared with the conventional method of 2 consecutive injections of PGF2 14 days apart, on some reproductive indices and concentrations of E2 and P4 on day 1, day 5 and 21 after insemination. All the cows assigned into the study were in their second parity onward and had no history of peri-parturient diseases including: retained placenta, dystocia, lameness, clinical mastitis and metritis. In the nPG group (n = 27), on day 40�5 postpartum, 2 doses of PG were injected 14 days apart and if after the 2nd injection, cow was inseminated upon showing signs of estrus. In the OC group (n = 25), Ovsynch-CIDR protocol along with timed AI was applied. Milk and blood samples were obtained from all the cows on day1, day 5 and 21 post AI. E2 and P4 of the plasma and P4 of the milk samples were measured by ELISA and reproductive indices were calculated. In case of return to estrus, the program was repeated for up to 3 times. Conception Rate (CR) was lower in the nPG than OC but the difference was not significant (p>0.05). In the 2nd and 3rd AI, CR was higher in OC than nPG (71.42 and 85.71% Vs 23.8 and 61.9% in the OC and nPG, respectively, p<0.05). Number of AI/conception and overall CR were 3.13, 40.3, 2.23 and 55.71% for nPG and OC, respectively (p<0.05). Days Open (DO) and predicted calving intervals were 138 and 418 and 106 and 387 for nPG and OC, respectively (p<0.05). Milk P4 was higher on day 5 and 21 in the OC and plasma E2 was lower in OC on day 5 than nPG (p<0.05). Results show that, ovulation synchronization by Ovsynch-CIDR, as compared with 2 consecutive PG injections reduced DO, increased CR in the 2nd and 3rd inseminations, increased overall CR and reduced number of inseminations per conception. It can not be claimed that results of this experiment might be similar in all the cows of the same herd or other herds because, cows assigned into this study were selected.
A.H. Fallah Rad and G. Ajam , 2008. Application of Ovsynch-CIDR vs 2 Consecutive Injections of PGF 2 , 14 Days Apart in Dairy Holstein Cows and Comparison of Reproductive Parameters, Plasma and Milk E2 and P4 Concentrations. Journal of Animal and Veterinary Advances, 7: 312-315.