Abstract: Laparoscopic partial splenectomy was performed in18 local breed dogs of both sexes divided into 3 groups (A-C) of 6 dogs in each. In group A, the dogs underwent laparoscopic partial splenectomy with adrenaline injection in the parenchyma of the spleen and titanium clips application on the stump in group B, partial splenectomy performed with a new laparoscopic thermocautery device, while group C, the same procedure as in group A, except the application of intracorporeal suturing on the stump instead of titanium clips. All the operations were performed under general anesthesia using Ketamine- xylazine combination. Three ports were induced on the abdominal wall, one for the telescope insertion, while the remaining 2 ports for insertion of the laparoscopic instruments. The gross and histopathological changes was studied in the 3 groups at 15 and 30 postoperative days. Titanium clips in group A, highly efficient in controlling the bleeding from the stump in addition of adrenaline injection, the time needed for this technique was (30±4.6 min). An excellent result was achieved by the new thermocautery device in group B, the device produced coagulation and partial cutting of the spleen without the need of blood vessel ligation or adrenaline injection, no bleeding was observed from the stump so, it does not need the application of clips or suturing, thermocautery technique consume less time than group A and C (22.5±4.6 min). In group C, the operation consumed the longest time when compared with group A and B (74±4.7 min), that most of the time was needed for the intracorporeal suturing of the stump. The only gross pathological change observed in the 3 groups that of omental adhesion with the stump. The histopathology revealed space formation at the site of clips application in group A and the formation of large necrotic area in group B due burning of tissue produced by the thermocautery, while in group C, presence of large nodules of fibrosis surrounded the suture material.