Authors : A. Rastegari, H. Roshanfekr, M. Mamouie and S.R. Khatami
Abstract: Growth Hormone is a peptide hormone synthesized by lactotropes of the anterior pituitary. It is well known that it playes an important role in biological processes such as mammary development, lactation, growth and metabolism regulation, being therefore a promising candidate gene marker for improving milk and meat production in cattle. Genomic DNA was isolated from blood samples of 84 Najdi cattle. A 211 bp GH gene exon IV segment was amplified by PCR using bovine specific primers. RFLP in this segment was studied using Alu1 restriction enzyme. The frequencies of genotypes were as follows, 0.6905 LL, 0.2857 LV, 0.2380 VV, frequencies of allele L and V were 0.8333 and 0.1667 in Najdi cows.
A. Rastegari, H. Roshanfekr, M. Mamouie and S.R. Khatami, 2010. Growth Hormone Genotyping of Najdi Cattle Breed Using PCR-RFLP. Journal of Animal and Veterinary Advances, 9: 1265-1266.