Abstract: The objective of this study was to determine the protein requirement for the maintenance of male Thai native cattle. About 18 cattle with body weight of 125±4.9 kg were arranged in a randomized complete block design with 6 replications (block) and 4 treatments. Cattle were fed with dietary Crude Protein (CP) levels of 5.0, 8.0 and 11.0% CP with similar amounts of metabolizable energy. Dry matter, organic matter, crude protein and neutral detergent fiber digestibility increased (p<0.05) with increasing levels of CP. Moreover, Nitrogen (N) intake, N absorption and N retention increased (p<0.05) with increasing levels of CP. Prediction equation CP intake (g CP kg^-1 BW^0.75) with relation to average daily gain (ADG, g kg^-1 BW^0.75) was CP intake = 0.409 ADG + 4.22 (R² = 0.883, SE = 0.624, p<0.01, n = 18). From these equations, it can be explained that the CP requirement for the maintenance of male Thai native cattle is 4.22 gCP kg^-1 BW^0.75.