Journal of Animal and Veterinary Advances

Year: 2010
Volume: 9
Issue: 11
Page No. 1660 - 1663

Comparison of DNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP

Authors : Reza Nahavandi, Nader Asadzadeh, Adbdolreza Soleimani Farjam, S.M. Nurul Amin, Parivash Hafezamini and Arash Javanmard

Abstract: Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 animals from four breeds from two species of Iranian cattle populations in include Bos taurus (Sarabi, Golpayegani) and Bos indicus (Sistani, Taleshi) were genotyped for the Pit-1 HinfI and leptin Sau3AI polymorphisms by the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The genotype and gene frequencies for each group were determined and shown to be quite variable among the breeds. The allele B for the leptin gene and allele A for the Pit-1 gene were investigated high frequency in Bos indicus. Candidate gene approach may be a useful method to measure of genetic distance for cross breeding program between taurin and indicine cattle.

How to cite this article:

Reza Nahavandi, Nader Asadzadeh, Adbdolreza Soleimani Farjam, S.M. Nurul Amin, Parivash Hafezamini and Arash Javanmard, 2010. Comparison of DNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP. Journal of Animal and Veterinary Advances, 9: 1660-1663.

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