Journal of Animal and Veterinary Advances

Year: 2010
Volume: 9
Issue: 21
Page No. 2759 - 2762

Using Bioinpormcotics Methods to Develop EST-SSR Makers from Sheep’s ESTs

Authors : Wenxiang Zhang, Zunbao Wang, Zongsheng Zhao, Xiancun Zeng, Hongbin Wu and Peng Yu

Abstract: About 29685 ESTs of sheep’s skin from NCBI were mined to develop EST-SSR markers. Totally, 1456 SSRs were identified from 1364 ESTs using SSRHunter soft tools. The frequency of EST-SSRs was 4.9%. The dinucleotide repeat motif was the most abundant SSR accounting for 32.14% followed by 28.23, 7.14, 25.21 and 7.28%, respectively for tri, hexa, penta and tetra-nucleotide repeats. Among the di-nucleotide repeats, AC/TG (58.33%) were the most abundant type. About 261 primer pairs were designed from microsatellite among which 62 pairs could show clear PCR products by electrophoresisand 5 of these primers revealed moderate to high Polymorphism Information Content (PIC) in Chinese Merino sheep. The mean PIC value was 0.6971, ranging from 0.5499-0.7440.

How to cite this article:

Wenxiang Zhang, Zunbao Wang, Zongsheng Zhao, Xiancun Zeng, Hongbin Wu and Peng Yu , 2010. Using Bioinpormcotics Methods to Develop EST-SSR Makers from Sheep’s ESTs. Journal of Animal and Veterinary Advances, 9: 2759-2762.

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