Authors : Tian Zhi-Quan, Wu Xiao-Wei, Sun Min, Yang Hai-Yan, Chang Guo-Bing, Ren Li-Wei and Li Bi-Chun
Abstract: The aim of this study was to examine Single Nucleotide Polymorphism (SNP) of resistant sequence in Mx gene in chicken with a mismatched Polymerase Chain Reaction-restriction Fragment Length Polymorphism (PCR-RFLP) method and thus, the differences of frequency on sequence A2032G in Mx gene in different breeds were detected. The results showed that three genotypes were found on A2032G in 15 breeds of chicken, which were AA, AG, GG and AA genotype was not found in Luyuan, Langshan chicken and Zhongyuan Game chicken, as GG genotype did in Chahua chicken and Red Jungle Fowl. The average frequency of resistance gene (A) and sensitive gene (G) was 0.350 and 0.650, respectively. However, the frequency of A alleles had a wide rangeability from 0.034-0.984 and the results of χ2-test indicated that Mx gene in all populations was in Hardy-Weinberg equilibrium.
Tian Zhi-Quan, Wu Xiao-Wei, Sun Min, Yang Hai-Yan, Chang Guo-Bing, Ren Li-Wei and Li Bi-Chun, 2010. The Genetic Distribution and Polymorphism Analysis of Antiviral Resistant Mx Gene Locus in Fifteen Chinese Indigenous Chicken Breeds. Journal of Animal and Veterinary Advances, 9: 402-405.