Authors : Li Yu, Chen Rong, Xu Da-Wen, Sun Pei, Xie Qian, Wei Jian-Zhong and Yin Zong-Jun
Abstract: To understand the molecular characteristics in PCV2 strains from Anhui province in China, the whole genomic sequences of the 6 PCV2 Anhui isolates which were associated with PMWS were cloning, sequencing and analyzing. The results show that full genomic sequences of all of 6 PCV2 Anhui isolates were 1767 nt in length and belong to the different clusters in the PCV2b, PCV2-1A, PCV2-1B, PCV2-1C. The genome of 6 PCV2 Anhui isolates have 99.5-99.7% homology in whole nucleotide sequence have 97.2-99.8% homology in ORF1 nucleotide sequence and 93.6-99.9% homology in ORF2 nucleotide sequence and have 97.0-100% homology in ORF2 amino acid sequence. About 6 PCV2, Anhui isolates have the 93-99.5% homology in the whole nucleotide sequence, 96.2-100% in ORF1, 89.8-100% in ORF2 with 42 strains of domestic and foreign. About 30 PCV2b isolates having the identity of 93.2-100% in ORF2 nucleotide sequence and 91.0-100% in ORF2 amino acid sequence. Researchers conclude that PCV2 genome nucleotide sequences were more stable and do not have stronger variations of different characteristics with the change of time and regions. PCV2 strains in Anhui region are close relatives but showed some of the regional characteristics; the variation was mainly concentrated in PCV2 ORF2. PCV2-1C gene cluster have changed in the ORF2 of the two main strains of the immune response zone.
Li Yu, Chen Rong, Xu Da-Wen, Sun Pei, Xie Qian, Wei Jian-Zhong and Yin Zong-Jun, 2011. Genetic Characterization of Porcine Circovirus Type 2 (PCV2) from Pigs in Auhui Province. Journal of Animal and Veterinary Advances, 10: 1024-1031.