Authors : Jiang Caode and Liu Yonggang
Abstract: The complete CDS sequences of three duck genes-MJD1, RHOG and RB11A were amplified using RT-PCR based on the sequence information of the mouse or other vertebrates. Sequence analysis of these three genes revealed that the duck MJD1 gene encodes a protein of 352 amino acids and has high homology with the Machado-Joseph disease protein 1 homolog (MJD1) of four species-rat (85%), human (87%), mouse (86%) and chicken (76%). The duck RHOG gene encodes a protein of 191 amino acids and has high homology with the RhoG precursor (RHOG) of six species-pig (100%), human, mouse and rat (98%), zebrafish (79%) and chiken (81%). The duck RB11A gene encodes a protein of 216 amino acids and has high homology with the Ras-related protein Rab-11A (RB11A) of nine species-chicken (99%), rat, human, rabbit, pig, mouse, dog and orangutan (99%) and bovine (98%). The phylogenetic tree analysis revealed that the duck MJD1 has a closer genetic relationship with the MJD1 of human and the duck RHOG has closer genetic relationships with the RHOG of pig but the duck RB11A has a closer genetic relationship with the RB11A of chicken. The RT-PCR gene expression analysis indicated that the duck MJD1, RHOG and RB11A gene was differentially expressed in tissues including lung, pancreas, intestine, fat, heart, spleen, liver and muscle. The study established the primary foundation for further research on these three duck genes.
Jiang Caode and Liu Yonggang, 2011. cDNA Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Duck Genes MJD1, RHOG and RB11A. Journal of Animal and Veterinary Advances, 10: 1526-1532.