Abstract: In this study, blood and milk samples were collected from 500 cattle that were selected out of 1250 cattle by random sampling method (40% of the animals) and detected by their ear numbers, located in 5 different enterprises in Konya and around between the years 2009-2010 and not vaccinated with Bovine Viral Diarrhea (BVD). Serum samples prepared from these specimen (blood and milk) were studied for antibody presence using commercial indirect ELISA kits and white blood cell samples were studied for antigen presence by commercially obtained direct ELISA kit. As a result of ELISA applied to blood serum samples, out of 500 animals, 449 were detected positive, 6 doubtful and 45 negative. Seropositivity was detected between 80.68-100% on the basis of enterprises while it was at a rate of 89.80% regionwide. As a result of ELISA applied to milk serum samples, out of 500 animals, 442 were detected positive, 1 doubtful and 57 negative. Seropositivity was detected between 82.50-95.24% on the basis of enterprises while it was at a rate of 88.40% regionwide. At the first step of the virological part of the study, as a result of ELISA applied to detect BVDV antigen in white blood cell samples, antigen presence was detected in only 3 animals out of 500 (0.60%). In the second sampling done to detect whether these 3 animals that were antigen-positive and antibody-negative were persistently infected or not, antigen presence couldn’t be detected in white blood cell samples and these 3 animals were considered as acute-infected in terms of BVDV. Consequently, PI presence wasn’t detected for cattle in the region and BVDV infection was at a similar rate when compared to the serological study done previously. Besides, the fact that close results were obtained as a result of ELISA applied to blood and milk serum shows that using milk serum in serological tests could be preferred as an alternative method to blood serum since sampling is easy and cheap for the researcher.