Authors : Chang Guobin, Liu Xiangping, Liao Jing, Chen Rong, Luan Deqin, Zhang Ying, Dai Aiqing, Ma Teng, Zhou Wei, Wang Kehua and Chen Guohong
Abstract: This study was conducted to investigate the different temporal and spatial expression pattern of pax-7 gene in embryo stage and growth stage of chicken, aiming to explore associations between muscle development and molecular markers of pax-7 gene. Breast and leg muscle tissue were selected from 8, 16, 20 and 21 days chick embryo and 2, 4, 6, 8, 10, 12 weeks rugao chickens. This study performed SYBR Green real-time fluorescence quantitative PCR to detect the expression pattern of pax-7 gene. The results showed that the expression pattern of pax-7 gene was similar between breast and leg muscles which it is lower during chicken embryo and reached to the highest point at 8-10 weeks of age. Pax-7 gene was continual expressed during chicken embryo and postnatal development, not only expressed in the embryo stage. Finally, we reassess current models of early patterning based on the analysis of these dynamic spatiotemporal expression patterns.
Chang Guobin, Liu Xiangping, Liao Jing, Chen Rong, Luan Deqin, Zhang Ying, Dai Aiqing, Ma Teng, Zhou Wei, Wang Kehua and Chen Guohong, 2011. Temporal and Spatial Expression of the Pax-7 Gene During Chicken Embryo and Postnatal Development. Journal of Animal and Veterinary Advances, 10: 1785-1788.