Authors : Caiyun Fan, Jianbo Cheng, Jinlian Li, Qinghua Wang and Dugarjaviin Manglai
Abstract: In this study, partial sequence of horse DRD4 gene was cloned including intron 1, partial exon 1 and exon 2. And restriction endonuclease Stu I was used to analyze the polymorphism of the DRD4 gene sequences of 270 horses from six types including importing breed, cultivating breed and local breed. The products of endonuclease cutting were detected by 8% non-denatured polyacrylamide gel electrophoresis and showing in silver staining protocol.The result indicated restriction endonuclease Stu I showed polymorphism. Six kinds of genotypes were found in six populations which were controlled by three alleles. The results of Chi-square (χ2) test showed that genotypes of horse DRD4 gene in TB, SH, XN did not fit with Hardy-Weinberg equilibrium (p<0.05) but in WS, BH and WZ fit with Hardy-Weinberg equilibrium (p>0.05).
Caiyun Fan, Jianbo Cheng, Jinlian Li, Qinghua Wang and Dugarjaviin Manglai, 2011. Polymorphism Analysis of the Horse Dopamine Receptor D4 Gene (DRD4) Sequence. Journal of Animal and Veterinary Advances, 10: 1855-1858.