Authors : Zhihua Cai, Like Wang, Deyi Liu, Shenghe Li, Xunsheng Pang and Yuxin Cheng
Abstract: In this study, researchers chose the Interferon Regulatory Factor 6 (IRF6) gene as a candidate gene to evaluate its effect on porcine immune traits. Using the PCR-RFLP analysis and sequencing, a SNP (HQ026023:1383 G>C) in exon7 of IRF6 gene was detected. In addition, a further analysis of SNP genotypes associated with immune traits including IFN-γ and IL10 concentrations in serum was carried out in three pig breeds including Large White, Landraces and Chinese indigenous breed Dingyuan Black pig. Results showed that the SNP of IRF6 gene effects on level of IFN-γ and IL10 (day 20) in serum presented highly significances (p = 0.0264; p = 0.0238) and as for the ratio of IFN-γ to IL10 (day 20) in serum, the p-value was 0.0341 which also achieved a significant level. All the results could possibly indicate that the IRF6 gene should be regarded as a molecular marker gene for genetic selection associated with these immune traits in the pig breeding program.
Zhihua Cai, Like Wang, Deyi Liu, Shenghe Li, Xunsheng Pang and Yuxin Cheng, 2011. Polymorphism Identification of IRF6 Gene and it Associate with Immune Trait in Three Pig Populations. Journal of Animal and Veterinary Advances, 10: 2095-2098.