Authors : Jiang Caode, Liu Yonggang and Xia Xueshan
Abstract: The complete coding sequences of three sheep genes-ARL2, ARL3 and ARL4C were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Sequence analysis revealed that sheep ARL2 gene encodes a protein of 184 amino acids that shares high homology with the ADP-Ribosylation factor-like 2 (ARL2) proteins of 9 species-cattle (100%), pig (99%), human (99%), dog (98%), mouse (96%), rat (95%), western clawed frog (90%), African clawed frog (89%) and channel catfish (85%). The sheep ARL3 gene encodes a protein of 182 amino acids that shares high homology with the ADP-Ribosylation factor-like 3 (ARL3) proteins of 9 species-cattle (100%), pig (99%), horse (98%), human (98%), mouse (98%), rat (97%), African clawed frog (97%), red jungle fowl (96%) and zebrafish (94%). The sheep ARL4C gene encodes a protein of 192 amino acids that shares high homology with the ADP-Ribosylation factor-like 4C (ARL4C) proteins of 11 species-cattle (100%), human (100%), chimpanzee (100%), mouse (100%), taeniopygia guttata (98%), gray short-tailed opossum (98%), green anole (98%), atlantic salmon (96%), rainbow smelt (96%), zebrafish (95%) and African clawed frog (91%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302310, 100302311 and 100302312. The phylogenetic analysis revealed that the sheep ARL2 and ARL3 genes both have closer genetic relationships with the ARL2 and ARL3 genes of cattle. The sheep ARL4C gene has a closer genetic relationship with the ARL4C genes of cattle, human, chimpanzee and mouse. Tissue expression profile analysis was also carried out and results demonstrated that sheep ARL2, ARL3 and ARL4C genes were all generally but differentially expressed in detected tissues.
Jiang Caode, Liu Yonggang and Xia Xueshan, 2011. Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (Ovis aries) Genes-ARL2, ARL3 and ARL4C. Journal of Animal and Veterinary Advances, 10: 2772-2778.