Authors : Victor Riojas-Valdes, Priscila Carrillo-Trevino, Diana E. Zamora-Avila, Rogelio Ledezma-Torres, Juan J. Zarate-Ramos, Jose A. Salinas-Melendez, Ramiro Ramirez-Necoechea and Guillermo Davalos-Aranda
Abstract: The goal of the present study was to determine by molecular methods the existence of an association among alleles of the Leptin Receptor gene (LEP-R) and dorsal fat in pigs, taking as a fixed effect the genotype for the ryanodyn receptor gene (Ryr-1). Genotyping for both genes was achieved by Polymersase Chain Reaction-restriction Fragment Longitude Polymorphism (PCR-RFLP) using specific PCR primers and restriction enzymes. Polymorphisms of LEP-R were determined by amplification of a 2 kilobase (kb) pair DNA fragment and digestion with restriction enzyme Hpa II whereas for Ryr-1 gene the PCR product is a 659 base pair (bp) fragment followed by digestion with enzyme Bis HKA I. A total 192 animals were analyzed (F1) not including the parental 4 males and 26 females. For Ryr-1 gene it was found a distribution of 75.7% dominant homozygous (normal), 22.6% heterozygous (normal carrier) and 1.7% recessive homozygous (affected). Allele frequencies were 0.87 N (normal) and 0.13 n (mutant). For LEP-R gene the results indicate 3.2% AA, 29.5% for AB and 67.2% for BB. Allele frequencies were 0.18 A (negative for RFLP), 0.82 B (positive for RFLP). Statistical analyses indicate that LEP-R genotype had an influence on dorsal fat deposition in the studied population.
Victor Riojas-Valdes, Priscila Carrillo-Trevino, Diana E. Zamora-Avila, Rogelio Ledezma-Torres, Juan J. Zarate-Ramos, Jose A. Salinas-Melendez, Ramiro Ramirez-Necoechea and Guillermo Davalos-Aranda, 2011. Association among Leptin Receptor Gene (LEP-R) with Carcass Weight and Dorsal Fat in a Commercial Type Swine Population from Mexico. Journal of Animal and Veterinary Advances, 10: 3006-3009.