Authors : Xun Wang, Haifeng Liu, Ling Zhao, Han Dong, Zhengxin Yan, Hengmin Cui and Xuewei Li
Abstract: Constitutive Androstane Receptor (CAR) is one of members of the nuclear receptors superfamily which is involved in induction of drug metabolism and lipogenic pathway. In this study, the reseachers investigated whether the mRNA expression pattern of CAR in porcine preadipocyte is changed during the process of induced differentiation. Preadipocytes were aseptically taken from 3 days old piglet under general anesthesia and grown to near confluence. Postconfluent cells (Day 0) were further cultured in differentiation medium for 3 days. From day 3 onward, the cells were cultured in maintain-medium based on differentiation medium without roglitazone and IBMX until day 15. The total RNA was isolated on seven time points (i.e., days 0, 2, 4, 6, 8, 10 and 15). The mRNA expression changes of the CAR before and after porcine primary preadipocytes differentiation that acorss seven time points (i.e., days 0, 2, 4, 6, 8, 10 and 15) were measured using the quantitative RT-PCR method. In addition, the researchers also examined lipid accumulation and the mRNA expression of adpocyte differentiation markers (Such as PPARγ and RXRα) which confirmed the differentiation of preadipocytes to adipocytes. The results showed that the mRNA expression of CAR was up-regulated during porcine primary preadipocyte differentiation. There is the reason that the CAR may be involved in the process of preadipocyte differentiation and is a potential drug target for the treatment of obesity and diabetes.
Xun Wang, Haifeng Liu, Ling Zhao, Han Dong, Zhengxin Yan, Hengmin Cui and Xuewei Li, 2012. Constitutive Androstane Receptor is Upregulated during Porcine Primary Preadipocyte Differentiation. Journal of Animal and Veterinary Advances, 11: 103-107.