Abstract: Transforming Growth Factor beta 1 (TGF-β1) is a pleiotropic cytokine that affects diverse biologic processes. In this study, the goat TGF-β1 gene was cloned and sequenced with length of 1173 bp. The predicted peptide of goat TGF-β1 contained 390 amino acids and 29 amino acids signal peptide. Phylogenetic analysis of different species available in GenBankTM based on the complete amino acid sequences of TGF-β1 showed that the goat was clustered with sheep sistered to bovine. The TGF-β1 mature peptide including 112 amino acids was expressed with a fusion protein of approximately 32 kDa. The goat PBMCs were proliferative stimulated by recombinant TGF-β1 but the proliferation of PBMCs stimulated by ConA was inhibited under co-stimulation of recombinant TGF-β1. These results suggest that the goat recombinant TGF-β1 would play an important role in regulating the immune responses and sustaining the balance of organism physiological state.
C.C. Shang, Y.Q. Zhao , D.Y. Cao , Y.L. Yao , D.K. Chen and J. Luo , 2012. Cloning and Expression of Transforming Growth Factor Beta 1 of Guanzhong Dairy Goat and its Effect on Proliferation of Goat Peripheral Blood Mononuclear Cells (PBMCs). Journal of Animal and Veterinary Advances, 11: 1127-1134.