Authors : Hua-Ying Chen, Feng-Jie Li , Sheng-Guang Fu , Ruo-Min Jin and Guang-Tao Yao
Abstract: A simple, fast and sensitive HPLC Method was developed for the simultaneous quantification of phenacetin, dapson, omeprazole and chlorzoxazone, reflecting the activities of CYP1A2, CYP3A4, CYP2C19 and CYP2E1 isozyme, respectively. HPLC analyses were performed on a reverse phase C18 column (5 μm, 4.6x250 mm) operating at 30°C. The mobile phase consisted of a acetonitrile and phosphate buffer (pH 6.4-6.5, 34:66). The eluate was detected at 280 nm and the retention times for each compound were within 10 min. The method was validated over the concentration ranges 0.25-25 μg mL-1 for dapson, omeprazole and chlorzoxazone, 0.5-25 μg mL-1 for phenacetine. The intra and inter day precisions were 0.88-5.80 and 3.26-7.75%, respectively and the accuracy ranged from 94.17-103.74 and 93.45-107.08%. The Limit of Quantification (LOQ) was 0.25 μg mL-1 for dapson, omeprazole and chlorzoxazone, 0.5 μg mL-1 for phenacetin. The present method provided a robust, fast and sensitive analytical approach for the four cytochrome P450 probe drug cocktail.
Hua-Ying Chen, Feng-Jie Li , Sheng-Guang Fu , Ruo-Min Jin and Guang-Tao Yao , 2012. Development of Four-Probe Drugs Cocktail Approach Evaluating Major Drug Metabolizing Enzymes Activity. Journal of Animal and Veterinary Advances, 11: 1405-1410.