Authors : Hong Cao, Xiaofeng Tian, Yuanjing Xu, Xudong Wang, Wulin Tian, Ten Wei, Xuedong Fang and Guanghong Xie
Abstract: To screen specific polypeptides binding to hepatoma carcinoma cells by using C7CTM Phage Display Peptide Library and carry out biological identification on their binding capacity. HL-7702 were used as the negative subtractive cells and HepG2 were used to screen as the target cells, 60 positive phage clones were randomly selected and cell-based ELISA was carried out to identify their binding activities to HepG2 cells. The positive clones were subjected to sequencing analysis, the polypeptides were synthesized, immunochemical and immunofluorescence analysis were also carried out for further identification. After four rounds of screening, significant enriching of phages appeared on the target cells HepG2; ELISA and immunochemical analysis were carried out, 18 positive phage clones with high binding capacity to hepatoma carcinoma cells were obtained from the randomly selected 60 phage clones, after sequencing and immunofluorescence identification, two polypeptides were confirmed having high affinity and their amino acid sequences had no homology.
Hong Cao, Xiaofeng Tian, Yuanjing Xu, Xudong Wang, Wulin Tian, Ten Wei, Xuedong Fang and Guanghong Xie, 2012. Screening and Identification of Polypeptides Specifically Binding to Hepatoma Carcinoma Cells. Journal of Animal and Veterinary Advances, 11: 2166-2175.