Authors : Ruosong Yang, Jinqing Jiang, Peiyu Gao, Xingyou Liu and Jinyou Ma
Abstract: The aim of this study was to identify the Classical Swine Fever Virus (CSFV) strain in isolates collected from 14 provinces in China between 2010 and 2011 by analyzing the sequences of the E0 genes which are the major genes in CSFV. After collecting samples from pigs that have the clinical symptoms of a CSFV infection, researchers isolated viral RNA and obtained the E0 gene by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Researchers analyzed the sequences of the E0 gene to determine the CSFV strain that caused the epidemic in China during 2010-2011. Finally, the complete genome of the epidemic strain was determined by RT-PCR analysis. The epidemic CSFV strain which was named HEBZ/CA/2010 was isolated from clinical samples obtained from the Hebei province of China and is 12296 Nucleotides (nt) in length. It is composed of a 372-nt-long 5'-terminal Untranslated Region (UTR), an 11697-nt Open Reading Frame (ORF) that encodes a polyprotein containing 3898 amino acids (aa) and a 227-nt 3'-UTR. Researchers compared the sequences of the HEBZ/CA/2010 isolate with those of other known CSFV isolates obtained from GenBank. The complete genome of HEBZ/CA/2010 shared 83.5-96.3% identity with that of other reported strains at the nucleotide level. On the basis of the analysis, the isolated HEBZ/CA/2010 was thought to belong to the highly virulent isolate group. This finding is useful for the prevention of CSFV epidemics and the development of a vaccine in China furthermore it contributes to studying the worldwide spread of CSFV.
Ruosong Yang, Jinqing Jiang, Peiyu Gao, Xingyou Liu and Jinyou Ma, 2012. The Complete Genome of an Epidemic Strain of the Classical Swine Fever Virus Identified in China During 2010-2011. Journal of Animal and Veterinary Advances, 11: 2459-2464.