Abstract: The complete coding sequences of three sheep genes-RHOA, RCHY1 and RSU1 were amplified using the Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The sheep RHOA gene encodes a protein of 193 amino acids that shares high homology with the ras homolog gene family, member A (RHOA) proteins of fifteen species- cattle (100%), rat (99%), human (100%), mouse (99%), chicken (99%), dog (99%), turkey (99%), sumatran orangutan (99%), poephila guttata (99%), Atlantic salmon (97%), green anole (96%), rainbow trout (96%), African clawed frog (96%), zebrafish (96%) and Western clawed frog (95%). The sheep RCHY1 gene encodes a protein of 261 amino acids that shares high homology with the ring finger and CHY zinc finger domain containing 1 (RCHY1) proteins of nine species-cattle (94%), giant panda (92%), pig (91%), human (90%), white-tufted-ear marmoset (90%), chimpanzee (90%), rabbit (89%), mouse (86%) and rat (84%). The sheep RSU1 gene encodes a protein of 277 amino acids that shares high homology with the ras suppressor protein 1 (RSU1) proteins of twelve species-cattle (99%), chimpanzee (98%), dog (99%), human (98%), giant panda (98%), horse (98%), rabbit (98%), chicken (96%), mouse (97%), African clawed frog (94%), Western-clawed-frog (94%) and zebrafish (91%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302078, 100302546 and 100302548. The phylogenetic analysis revealed that the sheep RHOA, RCHY1 and RSU1 genes all have a closer genetic relationship with the RHOA, RCHY1 and RSU1 genes of cattle. Tissue expression profile analysis demonstrated that sheep RHOA, RCHY1 and RSU1 genes were all generally but differentially expressed in detected tissues.