Authors : Wen-Lin Zhou, Jin-Ru Cao, Ai-Hong Ye, Cheng-Liang Gong, Hong-Biao Weng, Li-Hua He, Ren-Yu Xue, Guang-Li Cao and Yong-Qiang Wang
Abstract: The non-toxic B subunit of Escherichia coli heat-labile enterotoxin (LTB) is a potent mucosal immunogen and immunoadjuvant for coadministered antigens. To obtain transformed silkworm cell line stably expressing LTB, researchers fused the LTB coding sequence, neomycin-resistance gene (NeoR) and gfp gene into piggyBac-based transponson vector and transduced into silkworm BmN cells. After screening against antibiotic G418, the positive rate of cells emitting green fluorescence was 70.79%. PCR detection indicates the existence of exogenous LTB coding sequence, NeoR and gfp in the transformed cell genome. Western blot analysis also confirms the predicated ~60 kDa band of LTB protein. These results demonstrated that the strategy was practicable.
Wen-Lin Zhou, Jin-Ru Cao, Ai-Hong Ye, Cheng-Liang Gong, Hong-Biao Weng, Li-Hua He, Ren-Yu Xue, Guang-Li Cao and Yong-Qiang Wang, 2012. Expression of the B Subunit of Escherichia coli Heat-Labile Enterotoxin in Transformed Bombyx mori BmN Cells. Journal of Animal and Veterinary Advances, 11: 3785-3791.