Authors : Hua Wu, Shan-Mei Wang, Gong-Zheng Hu, Yu-Shan Pan, Bao-Guang Liu and Zhi-Pei He
Abstract: Thirty two Escherichia coli isolates recovered from diseased ducks diagnosed with colibacillosis in China between 2010 and 2011 were characterized for detection of Extended-Spectrum β-Lactamases (ESBLs) by phenotypic screening and confirmatory test provided by the Clinical Laboratory Standards Institute and antimicrobial susceptibility profiles via agar disk diffusion and a broth doubling dilution method. Results showed 7 of the 32 E. coli isolates analyzed (21.87%) were found to be ESBL producers. The resistant rates of ESBL producers were >28.6% against third generation cephalosporins and semisynthetic penicillins and as high as 100% against amoxicillin, ampicillin and ceftiofur. All 32 isolates were sensitive to imipenem, meropenem and cefepime and the susceptible rates of all 32 isolates to β-lactam antibiotics in combination with enzyme inhibitors were higher than those of β-lactam antibiotics alone. Therefore, ESBL-producing isolates had multi-drug resistance and enzyme inhibitors can partially solve the drug resistance problem.
Hua Wu, Shan-Mei Wang, Gong-Zheng Hu, Yu-Shan Pan, Bao-Guang Liu and Zhi-Pei He, 2012. Phenotypic Detection and Drug Resistance Analysis of Extended-Spectrum β-Lactamases among Escherichia coli Isolates from Diseased Duck. Journal of Animal and Veterinary Advances, 11: 3870-3875.