Authors : Y.O. Abdinasir, F.F.A. Jesse, A.A. Saharee, S. Jasni, S. Khairani-Bejo and A.W. Haron
Abstract: The purpose of the study was to describe the systematic infections of caseous lymphadenitis in mice with special reference to clinical manifestation and pathogenesis. In this, 104 apparently healthy mice, 2-3 weeks of old were selected and divided into 3 groups, namely control, whole cell and exotoxin groups. Mice of whole groups were exposed intraperitoneally to 1 mL of the inoculums containing 109 Colony-Forming Unit (CFU)/mL of live C. pseudotuberculosis. Exotoxin group were infected intraperitoneally with a single dose of exotoxin extracted from C. pseudotuberculosis. Control group were exposed intraperitoneally to 1 mL of phosphate-buffered saline. Groups of exotoxin and whole cell challenged showed prominent clinical signs that were characteristic of CLA which included depression, anorexia, submandibular oedema, yellowish and bloody diarrhea, ruffled caot, eye discharges, poor general condition and occasionally partial tremor of hindquarters. In necropsy, visceral abscess was the condition recorded in experimentally infected mice. There were also congestion and hemorrhages in the lungs, liver, spleen, kidney and intestine. Microscopically, there were lesions in the form of tuberculous granuloma (caseating tubercule), presence of giant cells, infiltration of neutrophils and macrophages, degeneration, vacuolation (necrosis), hemorrhage and formation of microabscesses. The results of this study indicated that manipulation of the organism and its extracted exotoxin (PLD) in experimenatal animals (mice model) led to clinical signs and pathological alterations that were similar to those conducted on small ruminant research of experimentally nature.
Y.O. Abdinasir, F.F.A. Jesse, A.A. Saharee, S. Jasni, S. Khairani-Bejo and A.W. Haron, 2012. Clinico-Pathological Changes in Mice Following Experimental Infection with Whole Cell and Exotoxin (PLD) Extracted from C. pseudotuberculosis. Journal of Animal and Veterinary Advances, 11: 4064-4072.