Authors : Jianglong Yuan, Yong Jin, Bing Zhu, Wei Wang, Yu Ren, Haiqing Wu, Fei Hao, Mingtu Nuo, Muzi Jin, Zhigang Wang, Ming Chang, Dongjun Liu and Xudong Guo
Abstract: The efficiency of donor cell types in cashmere goat Somatic Cell Nuclear Transfer (SCNT) is undefined. In this study, SCNT was performed using Caprine fetal Fibroblast Cells (CFCs), Caprine Ear Fibroblast Cells (CEFCs) and Transgenic Caprine Fetal Fibroblast Cells (TCFCs) as donor cells to compare the influences of cell type, transgene and sex of nuclear donor fibroblast cells on the SCNT efficiency including fusion and cleavage rates of reconstructed embryos and the birth, postnatal survival and mortality rates of cloned kids. A total of 4,943 reconstructed embryos were obtained. Among them, 3,949 embryos were fused and 3,737 embryos were cultured in vitro leading to a total of 3,094 cleavage embryos. Furthermore, embryo transplantation analysis was conducted on 1,873 cloned embryos with relatively normal morphology. A total of 368 recipient goats were transplanted and 48 goats were born of which 35 goats survived.
Jianglong Yuan, Yong Jin, Bing Zhu, Wei Wang, Yu Ren, Haiqing Wu, Fei Hao, Mingtu Nuo, Muzi Jin, Zhigang Wang, Ming Chang, Dongjun Liu and Xudong Guo, 2012. Somatic Cell Nuclear Transfer Efficiency Associated with Donor Cell Types in the Cashmere Goat. Journal of Animal and Veterinary Advances, 11: 4578-4584.